Discussion Paragraph 1
At t=0 min., the average total fluorescence intensity of NIH 3T3 cells at the surface of the cell was relatively high due to the lack of time that the endosomes had to migrate to the interior of the cell. At t= 0, transferrin had just been introduced to the cellular environment, and were only able to quickly to bind to the receptors of the cell, which rest on the cell surface. After 30 min. of incubation, cells exhibited relatively similar average total fluorescence intensities on the surface and interior, demonstrating moderate amounts of migration of endosomes to the interior of the cell. This added time allowed for the ligand bound transferrin to be internalized via clathrin coated pits to coated vesicles and migrate deeper into the cell. After 90 min. of incubation, the interior of the cell exhibits a relative higher average total intensity compared to the surface. This again is due to the added incubation time, which has allowed the previously early endosomes to acidify, releasing iron, which then brings the transferring molecules closer to the interior where sorting endosomes are found.
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