This project was aimed to asses the water quality and pollution levels of Sylvan stream using Periphyton. Periphyton are a class of aquatic microorganisms that grow in shallow slow moving water; they typically survive in water that has low to moderate pollution. We set up contraptions in three locations of the stream that would collect periphyton and we observed the populations under a microscope after one and two weeks. The first location had the least and we believe this is because it was the closest to campus and had higher pollution. The third spot fell in the middle and the second location had the most. Overall there was high amounts of growth on the slides so we're concluding that the stream does have moderate pollution, however the levels do increase closer to campus.