Denitrification Lab Results
For the Denitrification test, the results showed that there was nitrate present in both the rich and poor soil tubes because when nitrate reagent A and B were added to the Nitrate broth tubes, both turned red, meaning it is positive for nitrate. In addition, a small amount of gas was found in the durham tubes meaning nitrate was broken down into gaseous products.
For both Proteus vulgaris and Pseudomonas aeruginosa nitrate was broken down into gaseous products because there was a large amount of gas in the durham tubes. In addition, when zinc was added to the tubes, there was no color change meaning it was a positive result and nitrate was broken down into a compound other than nitrite such as nitrogen gas.