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Chemotaxis Motility Agar Lab

Submitted by mkomtangi on Fri, 04/06/2018 - 12:05

For the chemotaxis portion of the motility and chemotaxis lab, I hypothesized that the Serratia marcescens will move towards the nutrient source rather than the Enterococcus faecalis. I expect these results to occur because Serratia marcescens is a known motile organism and reacts in the presence of nutrients and therefore will be chemotactic, this same attraction will occur with the glycerol yeast extract (GYE). I began my experiment by collecting and labeling two petri dishes, I then added molten GYE to the label portions of my plates and let it solidify. After I added molten water agar into the halves of the petri dishes, again letting it solidify. I then put two pieces of filter paper on the plates creating a bridge for the bacteria to move throughout, I then inoculated the organism on each strip of paper at opposite points from each other, creating a diagonal cross.




An explanation of the organisms and what chemotaxis is would really add to the strength of paragraph.