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Endospore Hypothesis

Submitted by mkomtangi on Thu, 04/19/2018 - 17:35

After my observations, I found that my results were conclusive to my hypothesis. My results of the experiment showed that the pre-pasteurized NA plates contained lots of growth. Large colonies that seemed to be clumped together made it difficult to distinguish and count colonies, yet there seemed to have been one isolated colony present on the plate. The organisms appeared to have a dull texture with an opaque color, and slight elevation. As for the post-pasteurized plate, there was growth present. There was a lack of colonies present in comparison to the pre-pasteurized plate.


Both plates expressed similar colony morphology. Each colony that presented growth on the plate was isolated thus making counting possible. The organisms also presented an opaque color and very dull texture, with slight elevation. When observing the post-pasteurized organisms under the phase contrast microscope, the organisms were bacillus shaped in a chain-like arrangement with lots of movement. Tiny black dots within cells presented endospore formation, however no germination was detected. The post-pasteurized organisms that were gram stained and viewed under the light microscope at a total magnification of 1000x, expressed a  streptobacillus arrangement of organisms with distinct purple color indicating that the organisms were gram positive.