After having created a working map for our genomic DNA, identifying our gene of interest and doing extensive research on its possible functions, we needed to perform several experiments to find out more about our gene. The purpose of this lab was to use a reverse genetics approach to explore our Brachypodium distachyon gene of interest and its function by experimenting with mutant and wild type samples. We effectively worked from the mutant phenotype to explore the gene’s function. In doing so, we took several approaches to examine the phenotype, genomic DNA, phylogenetic relationships, and histology of the samples to identify the gene’s possible functions.
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