LLC-Pk1 parental cells will be cultured and plated in dishes. These cells will then be allowed to grow and divide until they are at confluent levels. Once the cells are confluent, a scratch assay will be conducted in which a small portion of the cells are scraped from the plate, creating a small and consistent gap between two large patches of cells. The cells are then washed twice with HBS and submerged in non-CO2 media. They are then observed under phase microscopy at 10X magnification in a time-lapse fashion. The migration of the cells into the “wound” will then be captured and analyzed. Rate of cellular migration and length of time it took for the wound to close will be parameters analyzed.
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