It is necessary for cells to be able to communicate at both intracellular and extracellular levels in order to grow and survive in a given environment. This laboratory exercise investigates the mechanisms of cellular response to internal and external stimuli, with respect to calcium ion transport, using a variety of agents (ATP, bradykinin, vasopressin). Exploration to determine whether a particular stimulus mediates calcium transport via plasma membrane channels or via release from intracellular stores was conducted using ionomycin (a calcium ionophore) to gain further insight on both levels of cellular communication.
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