Although quantitative analysis was not possible for fixed NIH 3T3 cells treated with TMR-dextran and lysotracker. This could have occurred due to possible insufficient exposure time and binning combinations when capturing images, or insufficient incubation time and conditions for the lysotracker and TMR-dextran. However, based on previous studies and common science knowledge, we are able to infer what visual and quantitative data of this investigation should resemble. After 15 min. of incubation time, the average total intensity of the TMR-dextran should be high at the surface of the cell relative to the interior. This is due to the relative brevity of the incubation time, where has not been sufficient time for many of the dextran molecules to be invaginated into the cell’s luminal space. The interior images should also display a relative high intensity of lysotracker at this point. After 75 min. of incubation time, the average total intensity of the TMR-dextran should be high at the interior of the cell relative to the surface. This is because the cell has had sufficient time to invaginate a majority of the dextran molecules and form endosomes and protonate, bringing them into the cell’s luminal space and closer to lysosomes. At this point, the interior images should display high lysotracker total intensities and high dextran total intensities, which would confirm that the dextran migrated to the inner most part of the luminal space.
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