The process by which cells intake extracellular material from the environment is known as endocytosis, and can occur in both a receptor-mediated and non-specific manner. This laboratory exercise investigates both of these endpcytotic processes over time using fluorescence microscopy to observe endosome migration in fibroblast (NIH 3T3) and epithelial (LLC Pk1) cells treated with transferrin and TMR-dextran. Additionally, effects of nocodazole in LLC Pk1 cells was studied to explore the efficacy of vesicle motility along microtubules in cells with depolymerized tubulin.
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