Methods for poster

Melanoma (B16) and fibroblast cells (NIH 3T3) were placed on a heated plate at 37°C. Using phase contrast microscopy at 40X magnification, multi-D acquire in ImageJ was employed to create movies of snaps taken of the live cells every 5 seconds for 95 intervals, creating seven minute movies. Time-lapse data was then analyzed observationally and quantitatively.