Fluorescent stained phalloidin was used to directly label the actin cytoskeleton of both fibroblasts (NIH 3T3) and LLC-Pk1 cells. Images of each cell type were taken at 10X and 100X magnification under a fluorescent filter, and in phase contrast. In visually examining the images, it is clear there is a stark difference in actin cytoskeleton composition for each cell type. Figures 3B and 4B demonstrate the actin cytoskeleton of LLC-Pk1 cells as a localized group of filaments around the nucleus and more compact. Figures 3D and 4D depict the cytoskeleton of NIH 3T3 cells under fluorescence, which appear to extend away from the nucleus and are less compact.
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