Overall, this laboratory exercise demonstrated the major elements that effect imaging in fluorescence microscopy. The net local concentration and degree of overlap of fluorophores and amount of the fluorescent dying in certain areas greatly effects the ability to achieve a bright and clear fluorescent microscopic image. Additionally, important microscope parameters such as the neutral density filters, and shutter/exposure time of the sample can greatly affect the brightness of an image and the rate at which fluorescent light decays over time, which is important to control in order to uphold the integrity of a sample.
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