Draft Bio 477H results paragraph 2

Fluorescein labeled tubulin was then time-lapse imaged under several conditions: auto shutter, open shutter, and open shutter with neutral density filters. Data was analyzed graphically for all three conditions (Figure 3). Open shutter data was shown to have the highest initial intensity value and rate of decay (Table 2). Auto shutter data contained midrange values for both initial intensity and rate of decay (Table 2). Time lapse data with neutral density filters and an open shutter demonstrated the lowest values for initial intensity and rate of decay (Table 2). Selected time lapse images visually demonstrate the difference in excitation intensity over time during the photobleaching process under all three conditions (Figure 4).