Bioimaging 477H Lab Report Results Section 1

Images of LLC-Pk1 pig kidney epithelial cells were taken under three different fluorescence filters in order to capture cellular structures stained with different fluorophores in the same region (Figure 1). These images were then superimposed on one another to create a comprehensive display of three cellular elements; dsDNA stained with DAPI (blue), tubulin stained with fluorescein (green), and f-actin stained with rhodamine (red). DAPI and fluorescein fluorophores showed visible fluorescence intensity and were captured in the image. The rhodamine stained f-actin did not appear in the composite image. Reasons for this disappearance is detailed in the Discussion.