Intramyocellular lipid content is a source of energy used within skeletal muscle. In this study, EM images of the vastus lateralis were taken for twenty four subjects who were matched for physical exercise. These EM Images were analyzed for IMCL content using GIMP and ImageJ programs. Based on the analysis we found that there was a age by sex difference in IMCL lipid area older women were found to have a much lower IMCL lipid area than in younger women.
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