Yeast Crosses

A clean adenine infused agar gel plate was then divided into four sections and labeled with the crosses that would be carried out. The quadrants were labeled HB1xHA0, HB1xHA1, HA1xHA2, and HB1xHA2. Small amounts of the two strains for each quadrant were mixed together without cross contamination and spread into small squares. The plate was then incubated at 30oC for a week. At the end of the week, all crosses had grown and displayed a cream-colored phenotype.