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Submitted by mglater on Wed, 04/25/2018 - 00:53

As expected, the H0 yeast matings served as controls, all resulting in white colonies. The H0 strain is unmutated, so the genes complemented any mutant that they were crossed with. Both of the unknown A type mutants gave the same results, meaning they share the same mutation. When crossed with HB2, they formed a white colony, meaning the genes complement each other. This means that the mutation in the A mutants is in Ade1. This is confirmed by the fact that the cross between the unknown mutants and HB1 results in little to no cell survival. The mutations are in the same gene, and therefore cannot complement each other. The two unknown alpha mutants also gave the same cross results and are therefore the same mutation. The unknown alpha mutants complemented with HA1 to produce a white colony, while they failed to complement with HA2. Following the same logic as with the A type mutants, this means that the mutation in the alpha yeast is in Ade2. Looking at the crosses between the A and alpha unknown mutants further supports this claim. The crosses between the unknown mutants all resulted in surviving white colonies, meaning that the genes complement. The only way for the mutations to complement are if the mutations are on different genes.