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Shh Tet-On

Submitted by mglater on Mon, 02/05/2018 - 16:27

Shh is a cell-cell signaling molecule that has been conserved between all vertebrates. Shh has been shown to be a vital part of driving cell differentiation in other areas of the body. This experiment will allow for a better understanding of the effect of sonic hedgehog in the developing, which will provide insight into the control of cellular development, the cell cycle, and of diseases such as cancer. This will be accomplished using the Tet-On system, a system designed to control transgenes. The genes are unable to be transcribed until the binding of tetracycline or a tetracycline derivative (such as doxycycline) to an activator protein. The addition of the doxycycline allows transcription to begin, giving very tight temporal control. The design of the experiment is to observe the effects of activating the transgene at different time points, ranging from 4 hours post fertilization (hpf) to 48 hpf.