Live LLC-Pk1 cells with GFP tagged tubulin were treated with TMR-dextran. Control group cells were incubated with solely TMR-dextran, while treatment group cells were incubated with TMR-dextran, and subsequently incubated in nocodazole. Both groups were time-lapse imaged at 40X magnification for 300 s (1 frame every 10 s). Vesicle movement was then quantitatively analyzed using ImageJ’s “TrackMate” plugin to measure total displacement of vesicles (Figure 5). Cells treated with nocodazole demonstrated a lower average displacement of vesicles compared to control group cells (0.164µm, 0.716µm, respectively) (Table 1). Composite image frames of start, middle, and end of the time-lapse image series visually display the differences in movement of the vesicles over time across groups (Figure 4).
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