Procedure for Trymyristin Isolation

1 gram of nutmeg was added to a round bottom Erlenmeyer flask and then 3 mL of TBME was added to the flask as well. The distillation process was then followed proceeded by a micro scale filtration that occurred pipet-full by pipet-full. 2 mL of TBME was then added again to the nutmeg and the filtration method was repeated. A light air stream was then run over the crude product and after a 5 minute drying period the weight was obtained. 12 mL of acetone was then added to the flask the solid was dissolved via a recrystallization process. The dissolved solution was cooled at room temperature and in an ice bath, the vacuum filtration method was then followed and the crystals were allowed to dry. A final weight and MP was then obtained. Following the filtration .072g of Trymyristin was then transferred to a round bottom flask. 2 mL of 6 M NaOH, 2 mL of 95% Ethanol and boiling chips were added to the flask. The solution was then refluxed for approximately 45 minutes. The remaining Trymyristin was then recrystallized and filtered a second time. The MP and final weight of the Trymyristin was recorded. After 45 minutes the refluxed solution was cooled at room temperature and transferred to a 50 mL beaker containing 8 mL of water. 2 mL of concentrated HCl was then added to the solution. The beaker was then cooled on ice while being stirred. The solid was collected via vacuum filtration and was allowed to dry overnight before obtaining a final weight and MP.