Indirect immunofluorescence staining and the direct labeling of components within the cell are two ways in which one can visualize parts of the cell that are otherwise too microscopic or transparent to identify. In this lab, we explore how primary antibodies impact efficacy of targeted cellular structure staining with respect to indirect immunofluorescence. Additionally, phalloidin coupled with fluorescent dye is used to directly label the actin cytoskeleton of two different cell types in order to visualize and investigate the organizational differences between the two cell types’ actin cytoskeleton structure.
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