In both the prokaryotic and eukaryotic cells glycolysis occurs in the cytoplasm of the cells. Although in the eukaryotic cell the formation of acetyl-CoA, the Citric Acid Cycle (Krebs) and the oxidative Phosphorylation occurs in an organelle called the Mitochondria. The opposite of breaking down glucose is gluconeogenesis, which forms glucose from pyruvate. In the prokaryotic cell the process of the formation of glucose occurs in the cytoplasm of the cell. In the eukaryotic cell gluconeogenesis starts in the mitochondria and finishes in the cytoplasm of the cell.
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