Photobleaching of LLC-Pk1 pig kidney epithelial cells was done with an open shutter and no neutral density filters for all three types of fluorophores. Time-lapse imaging data was collected and analyzed graphically for all three fluorophores (Figure 2). It is apparent that the DAPI labeled dsDNA has the highest initial intensity, and fastest rate of decay (Table 1). The rhodamine labeled f-actin holds both the lowest initial intensity value and rate of decay (Table 1). Fluorescein labeled tubulin have midrange values for both initial intensity and rate of decay (Table 1).
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