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Bio383H Lab 1 intro p. 3 draft

Submitted by liamharvey on Thu, 02/01/2018 - 19:23

After DNA had been extracted and degradation prevented, the DNA sample had to be purified. Protens, carbohydrates, and lipids were all present in solution and had to be removed for a pure DNA sample. Because carbohydrates and proteins are insoluble in solutions containing high concentration of potassium acetate, KOAc was used to separate these macromolecules. The macromolecules formed a pellet when in KOAc solution which was then removed. To rid the solution of lipids, an alcohol solution of isopropanol was used which formed the DNA into a pellet separate from the lipids. The pellet was then separated from the lipid solution and rinsed with EtOH.