Bio383H Lab 1 intro p. 3 draft

After DNA had been extracted and degradation prevented, the DNA sample had to be purified. Protens, carbohydrates, and lipids were all present in solution and had to be removed for a pure DNA sample. Because carbohydrates and proteins are insoluble in solutions containing high concentration of potassium acetate, KOAc was used to separate these macromolecules. The macromolecules formed a pellet when in KOAc solution which was then removed. To rid the solution of lipids, an alcohol solution of isopropanol was used which formed the DNA into a pellet separate from the lipids. The pellet was then separated from the lipid solution and rinsed with EtOH.