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Animal Hormone Lab Abstract

Submitted by benjaminburk on Mon, 04/23/2018 - 10:27

Fruit flies (Drosophila melanogaster) go through a 4-stage life cycle. A life cycle that is 11-14 days long providing an efficient option for observations in a laboratory setting (Potter 2000-2016).  This cycle can be altered simply by exposing the larvae to different hormones. We were eager to see the effect varying concentrations of Juvenile Hormone, a hormone essential to fruit fly development, inhibitor had on the life cycle of a fruit fly in a lab setting (Yamamoto R1, Bai H, Dolezal AG, Amdam G, Tatar M. 2013). Because of the fact that the juvenile hormone is essential to fruit fly development we hypothesize that the overall development will be stunted and predict to see a larger percentage of larvae in trials that contain a higher concentration of inhibitor and a lower percentage of pupae in trials that contain a higher concentration of inhibitor. We mixed 10ml of water (Control) or various concentrations (0.01, 0.1, and 1) Juvenile hormone (JH) Inhibitor with 2 grams of dehydrated fly media. We then added 2 male and 2 female flies to each tube. For a week we let the flies mate in the media. In the second week we removed all the adults from each trial and placed the tubes back into the incubator. After the third week we scored the vials for: number of larva, number of pupa, number of males and female adults, average length of adults. As you can see in Figure 1 there is an upwards trend seen in the percentage of larvae as the concentrations increase, as supported by the fact that 14% of flies in the 0.01 concentration were larvae and in the vial a concentration of 1 approximately 38.4% of the full population is larvae. Also in Figure 2 a downward trend is seen in the percentages of pupae, as supported by the fact that 63.4% of the population in 0.01 concentration were in the pupae stage, while the population with a concentration of 1 only had 35.1% of the population in the pupae stage.