383 lab 3 methods p 1

Using Phytozome, the original genomic sequence for our gene was searched in BLAST. The options Mutation Sites (v2) and Reference Sequence were selected.  Here the gene of interest was shown within the B. distachyon genome. Underneath the gene, several diamonds marked sites of mutations. Blue diamonds signified low impact mutations, yellow showed moderate and red showed high impact mutations. Five mutations within coding sequences were selected, including any high impact mutations. By clicking on each diamond, the mutation’s location was found. Zooming in enable the wild type and mutation reading frame to be seen and what change in sequence the mutation caused. The position and orientation of each mutation was added to our working map. A figure was then made on PowerPoint showing the position and mutation effect of each mutation relative to its location on the gene.