Results

Submitted by fmillanaj on Sun, 10/07/2018 - 17:15

    The significant difference between Figure 1 and Figure 1 is that the images contain a different amount of elements. Specifically, from Figure 2, a photo of the Lederle Graduate Research building is missing. This element was added specifically to highlight the location of the web in relation to how large the UMass campus is. Another noticeable difference is that the location of the spider-web is different. In the original figure, the spider web is very close to the ground, as can be seen by the sidewalk being a few inches below the spiderweb. The replica figure seems to have been found on a window. The spider web in the replica figure is also much bigger in size compared to its surroundings, while in the original figure, it can be assumed the spider web is much smaller due to how big its surroundings look compared to the web. The map in the original figure is noticeably much more zoomed in on the graduate building, as it contains much less of the buildings next to it. This is not true for the replica image, where multiple buildings can be seen, in addition to the Graduate building itself. The labeling is also different, wherein the original image, it is uppercase, standalone letters in a red font. In the replica image, the labels are inside gray boxes and use a different font/sizing.

 

CRISPR-Cas PP

Submitted by yurigarcia on Sun, 10/07/2018 - 03:54

One of the new techniques that has revolutionized the genetics field is CRISPR-Cas by cutting DNA. This important discovery provides a way of editing the genome that was applied to DNA in many organisms such as bacteria, fruit flies and yeast. There is an immunity that CRISPR-Cas has by being in bacteria and archaea and are used to defend these organisms against invaders. Scientists have invented a system known as CRISPR-Cas 9 that requires two RNA molecules, crRNA and tracrRNA. At the end it will facilitate the use of gene editing and by recognizing the specific sequence of DNA that works better than restriction enzymes. 

CRISPR-Cas draft

Submitted by yurigarcia on Sun, 10/07/2018 - 01:09

 

CRISPR-Cas is a new procedure that has transform the field of science, especially genetics that is used for cutting DNA. They occur naturally in bacteria and archaea and protects these organisms against anything that would harm them. A common system used in genetics is CRISPR-Cas9 that facilitates the use of gene editing. This system makes advances in recognizing a sequence of DNA than restriction enzymes.

Introcution, results for method draft

Submitted by jkswanson on Sat, 10/06/2018 - 16:57

Introduction

    The goal of this project is to create a multi panel scientific figure that is about a spider web, then write the methods of the procedure followed to create it.  Someone else will then follow the original methods and attempt to replicate the original image.

 Results

   Right off the bat the biggest difference seen is the web itself and the tree and are of the photo are all completely different.  The tree of the replicate is about 100 feet from the original tree. In figure A the webs themselves are different in size and visibility with the original being much larger and much more visible.  The original contains a dark purple folder behind the web to help it be more visible which is lacking in the replicate. There is also a leaf and other debris that are in the web in the original but not in the replicate.  The original image also contains a thumb and shouldve had a measuring tape at the bottom, but the editing messed it up, the replicate contains neither. Moving to figure A the map, they are different in size, the original being more zoomed in than the replicate.  The area circled in the replica is smaller than the original and black font is used as opposed to red in the original. The letters used to distinguish each figure are large black text in the original while the replica used drawn black letters. The positioning of these letters in the corner of each figure is different between the two figures.  In figure C the pictures are just different trees and areas. The entire panel of the original creates a tall rectangle that has each figure outlined in white. The replicat is not outlined and creates an elongated rectangle as the images are just placed in a row as opposed to figure A as a tall rectangle and B and C two squares stacked on top of eachother.  

 

Methods (rewritten)

Submitted by fmillanaj on Sat, 10/06/2018 - 13:55

To find a spiderweb on the UMass campus, I had to go through many trials and errors. The first spiderweb I found was too small for my phone to recognize. I had to go search for a bigger (more-defined) spider web. After a few days of searching, I found a spider web on the side of the Lederle Graduate Research center. On the section facing the main road (N Pleasant St), there was a spider web at about hip height. Photographing this web was quite difficult. I had to try to photograph it at several angles, with and without flash. I found that flash worked the best in making the web visible on in my photo. I had to angle the phone so that the camera was parallel to the main part of the spider web. (Addendum --- In addition to the spider web picture, I found a picture of the Lederle Graduate Research Center building (from the UMass Amherst website -- https://www.umass.edu/llc/lcc/lcc) on which the spider-web was on. This was to better show the location of the spider web in addition to providing a guide as to which side of the building the web was on.

    To create the figure, I gathered the location of the spider web on openmaps.eu, my photos of the spider web, and the picture of the Lederle Graduate building and put them in the inkscape app. I put the map on the top, the photo of the web on the bottom rights side, and the Graduate Building on the left bottom side. Then, I created labels to point out where the location of the spider web was, on both the map, and the pictures of where the web was. I labeled the map A in red font, the picture on the bottom right B in red font, and the picture on the bottom left C in red font. The organization of this was mainly to highlight the locations, starting from the furthest, the map to the closes, an actual picture of the spider web.

 

Psephurus and Polydon

Submitted by mtracy on Sat, 10/06/2018 - 00:08

Psephurus, or chinese paddlefish, is a large cartilaginous fish of the order acipenserifomres. These live in freshwater rivers, such as the Yangtze and its associated lakes. Historically, Psephurus could reach 7 meters in length. Unfortunately over time, these large fish were fished nearly out of existence. Additionally it was a major victim of the construction of the three gorges dam, as the migration that takens place between mating seasons could no longer take place. The last known specimen was killed illegally in 2007 and was only 3.6m long. No other Psephurus have been identified since.

The North American cousin to Psephurus is known as Polydon. Polydon lives in large freshwater rives as well, such as the Misissippi and Ohio rivers. Its long snout is covered with sensitive electrosensors, which can be used to detect swarms of plankton. The fish will swim forcefully through the water, injesting the plankton which are filtered through the gill rakes. Plankton are diverted to the fish's stomach, while water exits through the gill slits. Unlike Psesphurus, Polydon does not get as large; only about 2 meters in length. Unfortunately, much like Psesphurus, Polydon is a victim of overfishing, and due to dams can only be sustained by captive breeding.

Observations and Inferences

Submitted by yurigarcia on Fri, 10/05/2018 - 15:38

In the original picture the spiderweb is in the middle, the environment is in the middle and the map is on the right. On the replicate the spider web is on the left, the environment is on the middle and the map is on the right place. The arrow is pointing at the spiderweb on the original and in the replicate is pointing at the environment. The labeling letters in the original are in the picture and in the replicate are underneath of the pictures.  The original pictures are more zoom in and the replicate is taken from a far-away angle. Although it was taken in the same location it doesn’t show the spiderweb picture as the original. The maps are different in the original the ISB is emphasized and zoom in and in the replicate the map doesn’t specify the location.

Methods Draft

Submitted by jnduggan on Fri, 10/05/2018 - 13:48

The pictures themselves also differ from original to replicate.  Due to the quality of the pictures and lack of arrows on the environment picture of the replica, it is difficult to tell if the two spider webs are the same. The appearance of the stones relative to the spider web is different between the original and replicate in the close up picture.  In the environmental picture of the original figure, the curb and a blue building are visible in the background, but in the replica picture an orange building is visible and the curb is not. There is also a different number of posts on the fence visible in the replica vs. the original.

The pictures in the replica figure of the environment and close up have water on the stones and a relatively dark complexion, while the pictures of the environment and the spider web have a bright complexion and no water on the stones.  

 

discussion draft

Submitted by msalvucci on Fri, 10/05/2018 - 13:03

Observations of the replicated figure panel indicate many differences in the photographs between the two figure panels. The fact that the replicated picture shows a different amount of the tree next to the stair case suggests that the picture was taken a different angle than the original image. It was also observed that the picture showed more of the background of the spiderweb. From this difference, it is inferred that the photograph was taken from farther away in the replicate photo, thus, showing part of the student’s hands and more of the background behind the spiderweb.

The quality of the photographs taken are different between the original and replicated figure panels. The factor that’s most likely creating these differences in the lighting of the photographs would be the time of day that these pictures were taken.The weather also could be a factor affecting the picture quality, as the original pictures were taken on an overcast, cloudy day. This is most likely points to the camera exposure and focus.

The observed differences between the original and replicated figure panels are likely due to the in discrepancies in the methods. For example, the font size and type were different because the methods failed to completely describe the formatting. It is also inferred that a different version of Microsoft Word was used to create the replicate figure, as this would explain why the formatting differed.

 

Key draft

Submitted by eehardy on Fri, 10/05/2018 - 12:29
Key
A) Location of the Student Union on the Umass Campus Map. Student Union is circled in red. B) Front of the student Union. There are four different plants in front of the Student Union. The one of choice is circled in red. C) Plant pot on which the spider was located. Red arrows indicate the specific spot on the pot where the spider is located. D) Close up image of the spider.

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