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pp

Submitted by fmillanaj on Sun, 11/18/2018 - 18:32

E-site: the ribosomal site harbouring deacylated tRNA on transit out from the ribosome. Describe reading frame and then mention when the ribosome knows what is the correct reading frame . Starts with AUG in P-site

-mention direction of ribosome reads mRNA (3’ to 5’ or vice versa)

-then introduce tRNA and function. point out anticodons and anticodon loops

-use tRNA, amino acid , mRNA, and ribosome manipulatives

-form the peptide bonds(discuss the location of amino and carboxyl groups involved) and mention the dehydration reaction

-ribosome reaches stop codon and the disassembly of the ribosome and release of polypeptide and mRNA

-highlight important of termination

-discuss genetic code and exceptions

 

pp

Submitted by fmillanaj on Thu, 11/08/2018 - 17:46

A-site: the ribosomal site most frequently occupied by aminoacyl-tRNA. The aminoacyl-tRNA in the A-site functions as the acceptor for the growing protein during peptide bond formation.P-site: the ribosomal site most frequently occupied by peptidyl-tRNA, i.e. the tRNA carrying the growing peptide chain. The P-site is also referred to as the puromycin-sensitive site. Puromycin is an antibiotic which shows similarities with a part of aminoacyl-tRNA. When puromycin is present in the A-site, the peptide can be linked to puromycin via a peptide bond. Thus, peptidyl-tRNA in the P-site is located in the puromycin-sensitive site. E-site: the ribosomal site harboring deacylated tRNA on transit out from the ribosome.

methods

Submitted by fmillanaj on Thu, 11/08/2018 - 15:10

To determine the relationship between spider weight and spider web thickness, 3 different species of spiders of different sizes will be collected from various sites around campus. We will collect 2 spiders in each species. The spiders will be weighed on analytical scales and will be sorted based on weight. They will then be placed in separate containers  and will be allowed to spin out spider silk.The spiders will be left in their containers to make a web for 5 days. We will feed them 1 fruit fly each day. We will remove the web with tweezers and place onto a microscope slide. The silk will be fixed onto microscope slides using distilled water as a buffer. The silk will be observed under a microscope at 40x magnification and be characterized based on size, using a stage micrometer, and web type. The webs will be sorted based on type, and then subgroups will be made based on size. The data will be analyzed based on the spider species and then the web size will be compared to spider weight to determine if any correlation exists.

 

impact

Submitted by fmillanaj on Wed, 11/07/2018 - 22:57

Due to their extreme elasticity and ability to withstand remarkable forces, spider webs have a lot of potential in biotechnology. Discovering more about the factors that influence different characteristics of spider webs, like their thickness, can help us understand more about them and why they are so durable. Modeling the properties of spider webs could be useful in materials engineering and synthesizing a material that is both strong and soft. They could also be used for synthesizing replacement tissues that are flexible and strong.

 

virtual poster

Submitted by fmillanaj on Tue, 11/06/2018 - 20:00

Some things I noticed that either make a poster better or decrease its quality:

  • Adding too much information on the poster. I think this really distracts the reader and steers them away from important information. 
  • The organization is one of the most important traits for a poster. 
  • There are usually symbols that let the viewer know what affiliation the author has (university, company, etc…)
  • Pictures are important if they are relevant to the poster content.
  • I noticed that the design of the posters matters a lot more than I thought. 

 

synthesis

Submitted by fmillanaj on Mon, 11/05/2018 - 10:07

A-site: the ribosomal site most frequently occupied by aminoacyl-tRNA. The aminoacyl-tRNA in the A-site functions as the acceptor for the growing protein during peptide bond formation.P-site: the ribosomal site most frequently occupied by peptidyl-tRNA, i.e. the tRNA carrying the growing peptide chain. The P-site is also referred to as the puromycin-sensitive site. Puromycin is an antibiotic which shows similarities with a part of aminoacyl-tRNA. When puromycin is present in the A-site, the peptide can be linked to puromycin via a peptide bond. Thus, peptidyl-tRNA in the P-site is located in the puromycin-sensitive site. E-site: the ribosomal site harbouring deacylated tRNA on transit out from the ribosome.

 

project

Submitted by fmillanaj on Sun, 11/04/2018 - 18:22

    Spider webs are extremely thin, the largest silk fibers being only 5-10 μm. Some can be as thin as only 50 nm, making spider webs very difficult to measure. We found a publication that explains the use of light microscopy to accurately and precisely measure the width of spider webs. Webs are extremely strong, comparable to steel and also super flexible. However, because of the microscopic size, the different principles of silk have not been greatly investigated. In this review they used a polarized light microscope to illuminate the spider webs and images were taken that were then used for comparisons and measurements.

 

methods draft

Submitted by fmillanaj on Sat, 11/03/2018 - 18:57

To determine the relationship between spider weight and spider web thickness, 3 different species of spiders of different sizes will be collected from various sites around campus. We will collect 2 spiders in each species. The spiders will be weighed on analytical scales and will be sorted based on weight. They will then be placed in separate containers  and will be allowed to spin out spider silk.The spiders will be left in their containers to make a web for 5 days. We will feed them 1 fruit fly each day. We will remove the web with tweezers and place onto a microscope slide. The silk will be fixed onto microscope slides using distilled water as a buffer. The silk will be observed under a microscope at 40x magnification and be characterized based on size, using a stage micrometer, and web type. The webs will be sorted based on type, and then subgroups will be made based on size. The data will be analyzed based on the spider species and then the web size will be compared to spider weight to determine if any correlation exists.

 

draft

Submitted by fmillanaj on Fri, 11/02/2018 - 13:50

The law of definite proportions is a law of science that says that a chemical compound is always made up of the exact same proportion of elements by mass. So, if a compound is made by combining 1/4 from one chemical and the remaining 3/4 from another chemical, these proportions will always hold true, regardless of the number of chemicals added.

Pp

Submitted by fmillanaj on Fri, 11/02/2018 - 13:46

Protein is another macronutrient necessary to promote the health of the body. The body’s growth, repairing of cells,  and regulation of the body’s tissues and organs are all essential functions of the protein, therefore adequate amounts of protein in the diet is necessary to the optimal function of the body. My current food intake indicates a relatively steady amount of protein in my diet, at approximately 21 percent of the total calories I consume in one day. Overall, based on the two days of documenting my food intake, the main sources of protein are chicken, eggs, fish, and pork. The fish, unfortunately, was fried with batter, making a great source of protein that comes along with unnecessary amounts of fat, so in future meals I know to choose fish that’s steamed or cooked differently.The chicken is chicken breast and the pork was a lean piece of boneless pork chop, both relatively healthy options of protein, low in fat and sodium showing that the quality of the protein intake is quite well. Although those options of poultry and chicken are great, I could aim to expand my options and include beans, and nuts to obtain all the essential amino acids. Red meats, like beef, are excluded from being categorized as a healthy protein for it is much higher in saturated fats4. Harvard School of Public Health reported that the risk of dying from cardiovascular disease increased with increased consumptions of red meats, supported with evidence from a study that shows the risk of dying from cardiovascular increasing by 13 percent with every additional 3-ounce serving of red meat4.

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