Like I stated earlier, the first step in genotyping adult zebrafish is fin-clipping. By fin-clipping, we obtain tissue usable in DNA extraction. Each clipped fin should be placed in labelled PCR tubes. I'm going to discuss extraction using an extract-n-amp kit. To the PCR tubes, add 25µl extract and 6.25µl tissue prep, from the kit. Incubate at room temperature for 10minutes. Then, incubate at 950C for 3 minutes. Add 25µl neutralization buffer and dilute 1:10 in autoclaved double-distilled water. This 1:10 dilution will involve obtaining new tubes and adding 10µl ddH2O before adding 1µl of DNA extract from the original tubes. It is essential to extract DNA so that we can gain more insight into the genotypic nature of the adult zebrafish. After, we can run polymerase chain reactions (PCR) to isolate the gene of interest.
Comments
comment
"Like I stated earlier" is out of context and extraneous. Perhaps it is better to begin simply with "The first step in genotyping..." Another suggestion would be to avoid contractions like "I'm" in professional writing and use "I am."
Suggestion
I do not think you can have the word "then" in your writing because that turns the paragraph to be narrative rather scientific. Also, do not use words like "essential" because it is not objective term.
Comment
Try not to say "like i stated earlier" or "I'm going to discuss" this takes away from the flow of the paper and makes it less profrssional.