When defining our sample area, we chose areas of water that seemed to be as similar as possible. At the campus pond, we stood roughly one foot from the water, near an abundant source of vegetation including shrubbery and a tree. When we went to the Sylvan creek, we tried our best to find a location that matched the campus pond as best as possible. Eventually we found a spot that was surrounded by vegetation including shrubs, grasses, and trees, similar to those seen at the campus pond. To collect our samples from the two locations, we used a plankton net. The net was tossed roughly three meters into the water and reeled in. This process was repeated three times in order to gather as large of a sample as possible. Once this process was complete, the contents collected in the container attached to the net was transferred to a falcon tube and brought back to the lab in order to observe them underneath a microscope.