T-Blue and Ph-HCl staining

Toluidine blue (T-blue) stains polysaccharides and phloroglucinol HCL (Ph-HCL) stains lignin. In the NaN1793 mutants, both the Ph-HCL and T-blue stains appear a much lighter color at the distal cortex as opposed to the proximal cortex (Fig. 7B, 7D). In the wild - type plants, this contrast is not observed to as great of an extent (Fig. 7). The NaN1793 mutation is predicted to be a nonsense mutation, resulting in a truncated, nonfunctional protein. This phenotypic difference suggests that Bradi1g25180 may be involved in formation of cell walls of the distal cortex.