In this experiment, dog DNA was extracted and amplified through polymerase chain reaction (PCR). The DNA was then sequenced and analyzed using the Sanger Method. Excitability and size were chosen as traits to further analyze. By creating primers for dCAPS, the specific alleles for each trait were determined. The DNA was then dissolved with the digestive enzyme and ran on the gel for analysis. We had hypothesized that excitability and size are traits where heterozygous alleles exhibit codominance. Due to error in selection of the proper primer for creating our dCAPS, only the DNA marker was present on our gel. Therefore, no conclusion to our hypothesis was made.
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