By looking at the gels, we decided to extract M1 from H20 because it did not show up on TE. We then chose to take M2, M6, and M7 from the TE gel. After purifying the DNA from the gel using the gel extraction protocol. The concentration of M1 was 30.2 ng/uL. The concentration of M2 was 37.3 ng/uL. The concentration of M6 is 24.1 ng/uL. The concentration of M7 is 25.1 ng/uL. The A260/280 ratios are all near 2.0. This is usually considered pure for RNA. In this case, the fact that they are consistent across all four samples is a good indicator that we have pure DNA.
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