The goal of this experiment is to determine whether there is differential expression of the Bradi1g25180 gene between old versus young roots. Seeds were planted in gel and roots were grown for one week. New roots will be defined as the bottom half of the central axis root, while old roots will be defined as the top half of the central axis root. There are eight plants. There will be eight experiments; four of old roots and four of new roots. Each tube will contain either old or young roots from plants 1+2, 3+4, 5+6, or 7+8. The first step is to extract RNA. Once RNA is extracted and quantified using Nanodrop, RT-PCR will be performed. Primers that anneal to exons with an intron in between were made, such that the cDNA product is 261 b.p. long and the gDNA product is 537 b.p. long. This difference will allow us to see differential expression on a gel, because the more cDNA there is, the more RNA was present. High levels of RNA for a gene indicate high expression.
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