The first recrystallization of the crude trimyristin was performed using enough acetone to dissolve the crude product inside the Erlenmeyer flask. The mixture was cooled to room temperature for five minutes and left in the ice bath for 15 minutes, rinsed with additional acetone while collected via vacuum filtration. A weight and melting point was obtained. Hydrolysis was performed. A sample of the recrystallized trimyristin (0.06 g, 0.083 mmol) was transferred to a clean RB flask, along with 6 M NaOH (2.0 mL, 106.508 mmol), 95% ethanol (2.0 mL, 34.252 mmol), and two boiling chips. The solution was refluxed gently on the hot plate for 45 minutes. During hydrolysis, the remaining recrystallized trimyristin sample was recrystallized a second time in the same manner as before, except the warm solution was allowed to cool to room temperature for 10 minutes before cooling in ice for another 10 minutes. The weight and melting point were obtained. After the hydrolysis had proceeded for 45 minutes, the flask was allowed to cool to room temperature, after which the contents were poured into a 50 mL beaker containing water (8.0 mL, 444.0 mmol). HCl (2.0 mL, 65.826 mmol) was added dropwise while stirring until myristic acid precipitated. The beaker was cooled in ice water for 10 minutes, with stirring, and the resulting solid was collected via vacuum filtration, rinsing with water. A weight and melting point were obtained.
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