Gene Analysis Results 1

The ab initio program FGENESH predicted that the mRNA will be 2229 base pairs spanning 7 exons and the protein will be 742 amino acids long.  The homology-based program Phytozome predicted that the mRNA will be x base pairs and 847 amino acids long. The discrepancy between the two peptide sequences lies after amino acid 437 (Figure 2).  FGENESH predicts the following sequence to be KSLQ, while Phytozome predicts the sequence to be a set of 109 amino acids, nowhere containing KSLQ in sequential order. The sequence towards the C terminus of this discrepancy is in agreement.  21 ESTs were found in the Brachypodium distachyon genome with an Ident greater than 95%.  These ESTs were imported into CAP3 software to form contiguous sequences. Two contiguous sequences were created from the ESTs (Figure x and Figure x), and all ESTs contributed to the contigs; there were no singlets.  There is a very slights gap between the ESTs, but other than that they cover the whole cDNA found.