To find the official identity of the RZW gene, we performed a Phytozome BLAST search of the RZW genomic DNA with Brachypodium distachyon v3.1 as the target species. The best match to the query sequence was Bradi1g25180.1, and the predicted protein sequence was saved. The FGENESH and Phytozome predicted protein sequences were compared and the working map was updated. From the Phytozome locus page, the functional annotation was saved as a graphic showing domains. The link to Uniprot was followed and there were 16 annotations that contained information about different domains. We used NCBI BLAST to perform two Standard Nucleotide BLAST searches using the Nucleotide Collection (nr/nt) database and the Somewhat Similar Sequences (blastn) algorithm. Max target sequences was set to 1000, and the expect threshold was set to 1. The first search was of our genomic RZW sequence and the second was of the Phytozome predicted coding sequence. We used NCBI BLAST to perform a Standard Protein BLAST using the non-redundant protein sequences (nr) database and the protein-protein BLAST (blastp) algorithm. Max target sequences was set to 1000, and the expect threshold was set to 1.
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