The National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) was used as a homology-based method. We performed a Standard Nucleotide BLAST of the RZW genomic DNA using the Highly Similar Sequences (megablast) program in the Expressed Sequence Tags (EST) database for Brachypodium distachyon (taxid: 15368). All matches with greater than 95% Ident were saved. All of the saved ESTs were imported into the CAP3 software. CAP3 takes these and generated consensus contiguous sequences bases on the overlap between numerous ESTs. To find a full length cDNA sequence for our gene, we performed a Standard Nucleotide BLAST of the RZW genomic DNA using the Highly Similar Sequences (megablast) program in the Nucleotide Collection (nr/nt) database for Brachypodium distachyon (taxid: 15368). We found accession number XM_003562897.4, an mRNA sequence that is predicted to code for the Brachypodium distachyon G-type lectin S-receptor-like serine/threonine-protein kinase B120 (LOC100825184). The two contigs were aligned to the cDNA sequence using the NCBI BLAST.
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