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Zebrafish and Osmoregulation Results - Part 1

Submitted by sditelberg on Sun, 03/17/2019 - 23:17

Although statistical analyses have not yet been performed on these data, the Group 6 100 mM NaCl brain had higher average cell counts than those of the class in most regions of the brain, most noticeably in the hypothalamus and the telencephalon. This result alone matches the prediction that by altering salt conditions, cell proliferation rates will increase. This result alone also supports the hypothesis as well. It is crucial to note that Group 6 did not have a 50 mM NaCl brain to image and therefore could not accurately compare data with the class average for this salt concentration to draw any conclusions that may or may not have matched the prediction and supported the hypothesis. The system H2O and nanopure brains of Group 6 had slightly lower average cell counts than those of the class. It is important to note that during imaging of the nanopure brain, the telencephalon was cut off, therefore no accurate cell count could be obtained. These slight differences in cell counts for the system H2O and nanopure lines could be due to variation in counting as the greatest difference throughout individual regions of the brain is approximately 20 cells, exhibited in the lateral recess. For the system and nanopure lines, there is a difference of approximately 35 cells in the hypothalamus, yet this is a summation of the posterior and lateral recesses, which have differences of approximately 15 and 20 cells between Group 6 and the class averages, respectively. With other individual regions of the brain exhibiting these slight differences in counts as well, it is likely that the system H2O and nanopure lines vary due to counting or respective techniques.

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