I work in a research lab on campus. We work with anaerobic bacteria, meaning that they don’t require oxygen to make energy, in fact that bacteria that we use in my lab will die in the presence of oxygen. When we make media to put the bacteria in we must gas out the media. We typically do it by using a blend of nitrogen and CO2. To seal the bottle containing the media we place an air tight cap on called a bung. The bung must be place on the jar while the cannula (which is putting gas in the media) is still in the bottle. This task is incredibly hard to do considering the fact the bottle opening is barely big enough for the bung itself. In putting the bungs on I tore all the skin off the top of my middle finger. The wound is still healing almost one week later. It has a gross yellow scab which has grown over the top of it. The scab has begun to split and a slight hint of fresh blood has been coming out for the last couple days. I really need more calluses to form and I need them to form fast because everytime I create an environment for these bacteria to live in I will have to do this process.
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