This past week in lab, our aim was extracting DNA from two fish samples so we could analyze the DNA in PCR (polymerase chain reaction) and eventually gel electrophoresis. We started off by gathering our two fish samples, those being tuna and haddock. The samples were separately minced using razor blades and then transferred into two microcentrifuge tubes. Resuspension buffer was added to both tubes and lysis buffer was added. The tubes were inverted ten times and then placed in a water bath for ten minutes. Neutralization buffer was added and the tubes were then placed into the centrifuge for five minutes. The resulting supernatants were added to two spin columns that were placed in cap-less microcentrifuge tubes. Matrix beads were added and the samples were washed by pipetting distilled water into each tube and centrifuging. After three rounds, the final DNA sample was collected and stored until next week.
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