Draft 2/26

This is the expected results for my bacterial motility experiment.

Expected Results:

Motile organisms were expected to move from the point at which they were originally inoculated. The first way motility was tested was with a motility agar, this agar contains tetrazolium salt and will turn purple after it is oxidized. The two organisms tested were non-motile S. aureus and motile P. mirabilis. The agar inoculated with S. aureus was expected to only be purple at site of inoculation due to the fact it is non-motile and will not reduced the tetrazolium salt it is not immediately next to. The agar inoculated with P. mirabilis was expected to be entirely purple because it is a motile organism. This result would show that all tetrazolium salt had been reduced which means P. mirabilis moved to that part of the agar. The second test was to determine if an organized displayed taxis. The expected results were that S. marcescens would exhibit chemotaxis (the ability to detect and respond to different chemicals). Chemotaxis was expected to be displayed by the motile organism, in this case S. marcescens, by traveling from the nutrient poor water agar across a piece of filter to the nutrient rich GYE agar. E. faecalis is non-motile, it was expected to stay on the water agar and not grow due to the fact that it can not move nor does it display chemotaxis.