The budding yeast of Saccharomyces cerevisiae, a model organism, was chosen to analyze the effect of complementation within the adenine biosynthesis pathway. Four haploid strains were mated together and grown on MV plates. The resulting phenotypes of these crosses were then analyzed. Utilizing the phenomenon of genetic complementation helped determine whether the mutation causing an adenine deficiency was caused by the same gene in haploid yeast strains or by different genes. The HB1 strain was determined to be an α-strain with a genotype of ade- ,while strains HA0, HA1 and HA2 were determined to all be a-strains with HA0 being ADE+ and HA1 and HA2 being ade-.
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