To improve the quality and consistency of the experiment and cell counts across the class, the exact regions of the brain to be counted (e.g. telencephalon, posterior recess, etc.) could be encircled first. Implementing a standardized system for identifying regions of the brain is crucial to both accuracy and precision of experimental technique. This has already been partially started, but could be expanded upon and continued. Level of consistency in the brains obtained could also be improved. Because of low fish survival, salt-treated brains were obtained from a separate experiment, which may have led to differences in cell numbers, affecting the accuracy of the results. Perhaps less strong salt concentrations can be used when allowing embryos to grow to improve survivability. Salt-treated brains from the same experiment as the system H2O and nanopure brains can then be dissected and counted. Any group with extra salt-treated brains can give them to groups that do not have as many. Perhaps this may improve the accuracy of cell counting on the whole. Fixing protocol of the zebrafish larvae could also be changed to improve ease of dissection, allowing more time to run the experiment. After break, weaker salt concentrations could be experimented with and an improved protocol could be run, or a different variable could be tested. pH could be an interesting variable to explore with the zebrafish. Could slight changes in pH alter cell proliferation rates in the brain, and if so, would these changes occur in the same regions?
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