Six glass jars were gathered. The jars were divided into three groups consisting of two jars per group. The first group was labeled ‘Control,’ the second group was ‘Condition 1,’ and the third group was ‘Condition 2.’ Both ‘Control’ jars were each filled with 50mL of seawater. ‘Condition 1’ jars were each filled with 10mL of diesel and 40mL of seawater, while ‘Condition 2’ jars were filled with 25mL of diesel and 25mL of seawater. Using a micropipette, 1.5mL of sea urchin eggs were measured and released into each jar, as well as 1mL of dilute sea urchin sperm in each jar. After two minutes, each trial was stirred with separate pipettes and a drop from each was placed on six microscope slides with cover slips. They were examined under a compound microscope for a fertilization envelope. They were additionally viewed under a dissecting microscope. Afterwards, in intervals of 10 minutes, a sample from each trial was viewed under the compound and dissecting microscopes, to observe cleavage occurring and lysed eggs.
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