Methods of Jasmonic Acid experiment

After 14 days, 100 surviving plants were transplanted to pots and treatment fertilizer was added (Osmocote Classic 14-14-14, Scotts-Sierra Horticultural Products Company, Marysville, OH). Treatment of application of jasmonic acid and fertilizer amount was randomized, 50 plants were assigned treatment and 50 were not. The same soil used to plant the seeds was used. Half of the plants in each treatment group received one teaspoon of fertilizer, the other half received two. After application of fertilizer, plants were put onto greenhouse bench and watered. Plants were watered daily for 14 days. After 14 days, plant height was recorded on surviving plants. Non-surviving plants were discarded. Treatment of mechanical damage was done by cutting off half of some leaves and 1 spray jasmonic acid in acetone solution was applied. Control received 1 spray of acetone. Plants were placed on heat mat in greenhouse.