Like I stated earlier, the first step in genotyping adult zebrafish is fin-clipping. By fin-clipping, we obtain tissue we can use to extract the DNA. Each clipped fin should be placed in labelled PCR tubes. I'm going to discuss extraction using an extract-n-amp kit. To the PCR tubes, add 25µl extract and 6.25µl tissue prep, from the kit. Incubate at room temperature for 10minutes. Then, incubate at 950C for 3 minutes. Add 25µl neutralization buffer and dilute 1:10 in DEPC water. It is essential to extract DNA so that we can gain more insight into the genotypic nature of the adult zebrafish. After, we can run polymerase chain reactions(PCR) to isolate the gene of interest.
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