The ab initio protein prediction and the homology based protein prediction had a substitution that involved 4 amino acids becoming 140. Ab initio methods are purely based on theoretical trends of transcription and translation, while homology based methods compare the reference sequence to known sequences. I believe the homology-based protein prediction is more accurate. In both DNA and protein level homology searches, the “G-type lectin S-receptor-like serine/threonine-protein kinase” terminology was repeated in results with high levels of homology. This makes me confident to say that the Bradi1g25180.1 gene encodes a G-type lectin receptor kinase. However, the functions of many of these G-type lectin receptor kinases are not well characterized. Sun et al implicated an Arabadopsis thaliana G-type lectin receptor kinase called GsSRK to salt stress. They showed that is is upregulated when a plant is under salt stress, and also showed that its overexpression leads to increased salt tolerance. Unfortunately, they did not perform a knockout of this gene. To my knowledge, there are no papers that have studied a knockout mutation of a G-type lectin receptor kinase in any organism.
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