Our unknown gene’s protein sequence was compared for similarities to other proteins by performing a Protein BLAST with the same algorithm parameters as the previous search. The “nonredundant protein” database was searched. This search strategy was saved. In the output, matches that seem false were noted. Back at the blastp page, the searches were sequentially limited to: Brachypodium, plants, animals, insects, mammals, fungi, bacteria, archaebacteria, saving the search strategies every time. The extent to which our protein appears among living organisms was noted.
Library research of the unknown gene was performed, first by expanding the descriptions of the conserved domains on the NCBI Protein BLAST output. The Phytozome predicted amino acid sequence was then entered into the European Bioinformatics Institute website, and information and publications were found there. Further research was performed on the Strubbelig-Receptor Family 7 (SRF7) and 6 (SRF6) that were highly similar to our unknown gene.
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